JOSD1, Recombinant, Human, aa1-202, His-Tag (Josephin-1, dJ508I15.2)

Size

£512.00

SKU:: 138674
Molecular Weight:: 25.6
Purity:: ≥90% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM tris-HCl buffer, pH 8.0, 0.2M sodium chloride, 5mM DTT, 2mM EDTA, 40% glycerol.
Extra Details:: JOSD1, also as known as Josephin-1, has low protease activity towards poly-ubiquitin chains (in vitro). JOSD1 is act as a deubiquitinating enzyme. Recently identified ubiquitin-binding sites in the Josephin domain contribute to ubiquitin chain binding and cleavage. Source: Recombinant protein corresponding to aa1-202 from human JOSD1 at N-terminal, fused to His-tag expressed in E.coli. Molecular Weight: ~25.6kD (225aa) Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGS MVTPHKKSML GNGNYDVNVI MAALQTKGYE AVWWDKRRDV GVIALTNVMG FIMNLPSSLC WGPLKLPLKR QHWICVREVG GAYYNLDSKL MSCVPWK GDKAKSESLE LPQAAPPQIY HEKQRRELCA LHALNNVFQD SNAFTRDTLQ EIFQRLSPNT KMPEWIGGES ELRKFLKHHL RGKNCELLLV VPEEVEAHQS WRTDV Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.