ECSIT, Recombinant, Human, aa19-217, His-Tag (Evolutionarily Conserved Signaling Intermediate in Toll Pathway Mitochondrial, SITPEC)

Size

£607.00

SKU:: 138645
Molecular Weight:: 24.6
Purity:: ~90% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM tris-HCl buffer, pH 8.0, 0.1M sodium chloride,1mM DTT, 10% glycerol.
Extra Details:: ECSIT is a ubiquitously expressed protein that plays an important role as an adaptor protein in the cytosolic signal transduction cascade events triggered by Toll receptor activation. It was initially identified as a cytoplasmic protein interacting specifically with TNF receptor associated factor (TRAF)-6 in the TLR pathway. Knockdown of ECSIT results in severely impaired complex I assembly and disturbed mitochondrial function. Source: Recombinant protein corresponding to aa19-217 from human ECSIT at N-terminal, fused to His-tag expressed in E.coli. Molecular Weight: ~24.6kD (222aa) AA Sequence: MGSSHHHHHH SSGLVPRGSH MGS VYYHILRADL LPPEEREVEE TPEEWNLYYP MQLDLEYVRS GWDNYEFDIN EVEEGPVFAM CMAGAHDQAT MAKWIQGLQE TNPTLAQIPV VFRLAGSTRE LQTSSAGLEE PPLPEDHQEE DDNLQRQQQG QS GTCGAAL TGTSISQVPL PKDSTGAADP PQPHIVGIQS PDQQAALARH NPARPVFVEG PFSLWLRNKC Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.