CYTH2, Recombinant, Human, aa1-399, His-Tag (Cytohesin 2, ARNO, CTS18, CTS18.1, PSCD2, PSCD2L, SEC7L, Sec7p-L, Sec7p-like)

Size

£512.00

SKU:: 138642
Molecular Weight:: 48.9
Purity:: ≥90% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM tris-HCl buffer, pH 8.0, 0.2M sodium chloride,1mM DTT, 10% glycerol.
Extra Details:: CYTH2, also known as Cytohesin-2 and ARNO, is an ARF-1 guanine nucleotide exchange factor (GEF). ARF (ADP ribosylation factor) proteins, a group within the RAS superfamily, are GTP-binding proteins central to the process of vesicle budding. This protein promotes guanine-nucleotide exchange on ARF1, ARF3 and ARF6. Also it promotes the activation of ARF factors through replacement of GDP with GTP. The cell membrane form, in association with ARL4 proteins, recruits ARF6 to the plasma membrane. Source: Recombinant protein corresponding to aa1-399 from human CYTH2 at N-terminal, fused to His-tag expressed in E.coli. Molecular Weight: ~48.9kD (422aa) Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGS GRKKFNMDPK KGIQFLVENE LLQNTPEEIA RFLYKGEGLN KTAIGDYLGE REELNLAVLH AFVDLHEFTD LNLVQALRQF LWSFRLPGEA MEDGVYE PPDLTPEERM ELENIRRRKQ ELLVEIQRLR EELSEAMSEV EGLEANEGSK TLQRNRKMAM QKIDRMMEAF AQRYCLCNPG VFQSTDTCYV LSFAVIMLNT SLHNPNVRDK PGLERFVAMN RGINEGGDLP EELLRNLYDS IRNEPFKIPE DDGNDLTHTF FNPDREGWLL KLGGRVKTWK RRWFILTDNC LYYFEYTTDK EPRGIIPLEN LSIREVDDPR KPNCFELYIP NNKGQLIKAC KTEADGRVVE GNHMVYRISA PTQEEKDEWI KSIQAAVSVD PFYEMLAARK KRISVKKKQE QP Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice