138601-AF-ML650
UPK3A (Uroplakin-3A, UP3a, UPIIIa) (MaxLight 650)
Size
£876.00
- SKU:
- 138601-AF-ML650
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to aa260-279 (GASESSYTSVNRGPPLDRAE) from human UPK3A.
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
- Extra Details:
- MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. Uroplakin-3A (UPK3A) is a component of the asymmetric unit membrane (AUM). It is a highly specialized bio-membrane made by terminally differentiated urothelial cells. The protein may play an important role in AUM-cytoskeleton interaction in terminally differentiated urothelial cells. UPK3A also contributes to the formation of urothelial glycocalyx, which may play an important role in preventing bacterial adherence through FimH bacterial protein binding leading to bladder infection . UPK3A has been shown as a helpful marker for the detection of bladder cancer. Applications: Suitable for use in Western Blot, Flow Cytometry, Immunoprecipitation and Immunofluorescence. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1ug/ml Flow Cytometry: 0.5-1ug/10e6 cells Immunofluorescence: 1-2ug/ml Immunoprecipitation: 1-2ug/500ug protein lysate Optimal dilutions to be determined by the researcher. Positive Control: HEK2 93 cells or bladder carcinoma Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice