138572-AF-APC

MUC5AC (Apomucin Major Airway GlycoProtein, Mucin 5 subtype AC tracheobronchial, Mucin 5 Subtypes A And C, Mucin 5AC oligomeric mucus/gel forming, Tracheobronchial Mucin, TBM, Mucin 5AC, Gastric Mucin) (APC)

Size

£876.00

SKU:: 138572-AF-APC
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient
Clone:: 45M1
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Extra Details:: This monoclonal antibody recognizes the peptide core of gastric mucin M1 (>1,000kD) (recently identified as Mucin 5AC). Its epitope is destroyed by beta-mercaptoethanol and proteases but not by periodate treatment. Antibody to gastric mucin M1 reacts with the gastric epi thelium of normal human gastrointestinal tract as well as with the precancerous and cancerous colon but not w ith normal adult colon. It also reacts with fetal colonic mucosa. Resurgence of gastric mucin r eactivity during colonic carcinogenesis is due to re-expression of the peptide core of gastric (or fet al colonic) mucins. Applications: Suitable for use in FLISA and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (Formalin-fixed): Staining of formalin-fixed tissue requires boiling tissue sections in 10mM citrate buffer, pH 6, for 10-20mins followed by coooling at RT for 20mins. Optimal dilutions to be determined by the researcher. Positive Control: Stomach Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. APC is light sensitive. Note: Applications are based on unconjugated antibody.

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