138571-AF-PE

MUC-1 (EMA, Epithelial Membrane Antigen Mucin-1 ) (PE)

Size

£876.00

SKU:: 138571-AF-PE
Purity:: Purified by Protein A/G affinity chromatography.
Storage Conditions:: 4°C Do not freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human milk fat globule membrane (NP_001018016; P15941). Cellular Localization: cytoplasmic and cell surface
Clone:: GP1.4
Format:: Supplied as a liquid in PBS. No preservative added. BSA-Free. Labeled with R-Phycoerythrin (PE).
Extra Details:: The alpha subunit has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain, which is involved in cell signaling, through phosphorylation s and protein-protein interactions. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinoma tissues. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver. Applications: Suitable for use in FLISA, Flow Cytometry, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Positive Control: MCF-7 or MDA-231 cells. Breast, colon, ovarian or endometrial carcinoma. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

Shipping Conditions:: Blue Ice