138554-AF-ML490
Granulocyte (MaxLight 490)
Size
£876.00
- SKU:
- 138554-AF-ML490
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Nuclei from pokeweed mitogen stimulated human peripheral blood lymphocytes.
- Clone:
- BM-2
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
- Extra Details:
- MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. Recognizes an unidentified antigen in the cytoplasm of mat ure granulocytes. It shows no reactivity with any other cell type in human tissues. Markers of m yeloid cells are useful in the identification of different levels of cellular differentiation. Monoclonal antibody BM-2 reacts with early precursor and mature forms of human myeloid cells. This monoclonal antibody is useful for the detection o f myeloid leukemias and granulocytic sarcomas. It can be used as a marker of granulocytes in normal tissues or inflammatory processes. Applications: Suitable for use in FLISA, Flow Cytometry, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-1ug/10e6 cells Immunofluorescence: 1-2ug/ml Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT Optimal dilutions to be determined by the researcher. Positive Control: Tonsil or lymph node Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice