138554-AF-FITC
Granulocyte (FITC)
Size
£876.00
- SKU:
- 138554-AF-FITC
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Nuclei from pokeweed mitogen stimulated human peripheral blood lymphocytes.
- Clone:
- BM-2
- Format:
- Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with Fluorescein isothiocyanate (FITC).
- Extra Details:
- Recognizes an unidentified antigen in the cytoplasm of mat ure granulocytes. It shows no reactivity with any other cell type in human tissues. Markers of m yeloid cells are useful in the identification of different levels of cellular differentiation. Monoclonal antibody BM-2 reacts with early precursor and mature forms of human myeloid cells. This monoclonal antibody is useful for the detection o f myeloid leukemias and granulocytic sarcomas. It can be used as a marker of granulocytes in normal tissues or inflammatory processes. Applications: Suitable for use in FLISA, Flow Cytometry, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-1ug/10e6 cells Immunofluorescence: 1-2ug/ml Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT Optimal dilutions to be determined by the researcher. Positive Control: Tonsil or lymph node Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice