ERBB2 (HER-2, CD340)

Size

£422.00

SKU:: 138547
Purity:: Purified by Protein A/G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human HER-2. Cellular Localization: Cell Surface
Clone:: HRB2/451
Format:: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138547-AF.
Extra Details:: This monoclonal antibody is specific to c-erb B-2/HER-2 and shows minimal cross-reaction with other members of the family. C-erbB-2/HER-2 is a member of the EGFR family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erb B-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary. Applications: Suitable for use in Flow Cytometry and Immunofluorescence. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1.2ug/10e6 cells Immunofluorescence: 1-4ug/ml Optimal dilutions to be determined by the researcher. Positive Control: MCF-7, SKBR-3 Cells or Breast Cancers Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.