EpCAM (CD326, Epithelial Cell Adhesion Molecule, Epithelial Specific Antigen)

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£576.00

SKU:: 138543
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Small cell lung carcinoma cell line H69.
Clone:: VU-1D9
Format:: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Extra Details:: CD326 / EpCAM (also known as ESA, EGP40, EGP-2, KSA1/4, CO17-1A, GA733-2, MOC31, Ber-EP4) is a 40 kDa transmembrane glycoprotein serving as adhesion molecule in the basolateral membranes in a variety of epithelial cells. CD326 mediates calcium-independent homotypic cell-cell adhesions. CD326 over-expression has been detected in many epithelial tumours and is often associated with bad prognosis. It has been used for diagnostics of (pre-) malignancies at early stages. Applications: Suitable for use in Western Blot, Flow Cytometry, Immunocytochemistry, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (paraffin sections): 5-10ug/ml. Antigen retrieval with heat (sodium citrate) and trypsin. Immunohistochemistry: frozen Immunocytochemistry: 4ug/ml. Western Blot: 1-4ug/ml, under non-reducing conditions. Flow Cytometry: 1-3ug/ml Immunoprecipitation: 1-2ug/100-500ug of protein in 1ml lysate. Optimal dilutions to be determined by the researcher. Immunohistochemistry Positive Control: Colon epithelium Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice