Cytokeratin, HMW (High Molecular Weight Keratin)

Size

£422.00

SKU:: 138537
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human epidermal keratin
Clone:: AE-3
Format:: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138537-AF.
Extra Details:: This monoclonal antibody recognizes basic (Type II or HMW) cytokeratins, which include 67kD (CK1); 64kD (CK3); 59kD (CK4); 58kD (CK5); 56kD (CK6); 52kD (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40kD. Monoclonal antibody AE3 recognizes the 65-67, 64, 59, 58, 56, and 52kD keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. Applications: Suitable for use in ELISA, Immunofluorescence, Western Blot, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-1ug/10e6 cells Western Blot: 0.5-1ug/ml Immunofluorescence: 1-2ug/ml Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT Optimal dilutions to be determined by the researcher. Positive Control: Skin, Adenocarcinomas Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice