138530
CD8a (T-cell Surface Glycoprotein CD8 alpha Chain)
Size
£422.00
- SKU:
- 138530
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to aa200-250 from human CD8a.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138530-AF.
- Extra Details:
- CD8 is a cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cell express CD8a. CD8 binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells. For mature T-cells, CD4 and CD8 are mutually exclusive, so anti-CD8, generally used in conjunction with anti-CD4. It is a useful marker for distinguishing helper/inducer T-lymphocytes, and most peripheral T-cell lymphomas are CD4+/CD8-. Anaplastic large cell lymphoma is usually CD4+ and CD8-, and in T-lymphoblastic lymphoma/leukemia, CD4 and CD8 are often co-expressed. CD8 is also found in littoral cell angioma of the spleen. Applications: Suitable for use in ELISA, Western Blot, Immunofluorescence, Immunoprecipitation, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence: 1-2ug/ml Immunoprecipitation: 1-2ug/500ug protein lysate Flow Cytometry: 1-2ug/10e6 cells Western Blot: 1-2ug/ml Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 1-2ug/ml/30min @ RT Optimal dilutions to be determined by the researcher. Positive Control: HuT78 or hPBL. Tonsil Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice