138517-AF-ML490
CD45RA (Leukocyte Common Antigen, LCA) (MaxLight 490)
Size
£876.00
- SKU:
- 138517-AF-ML490
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Stimulated human leukocytes
- Clone:
- 158-4D3
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
- Extra Details:
- MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. CD45 (leukocyte common antigen), the most common hematopoietic lineage marker, is a transmembrane glycoprotein tyrosine phosphatase with important roles in immune system signal transduction pathways. Antibody panels have been instrumental in identifying multiple isoforms, including CD45RA. Isoforms differ in their extracellular domain and are generated by alternative splicing of exons 4, 5 and 6 encoding peptide segments called A, B, and C, respectively. At least five different isoforms have been identified in humans: containing all three (ABC), two (AB and BC), one (B) or no (O) exons. A given antibody recognizes either every isoform (CD45 antibody) or only a subset (“restricted” CD45R antibody). The CD45RA antibody clone reacts with the ABC and BC isoforms (Schlossman et al, 1995). CD45RA has a molecular weight of 205-220kD. Isoform expression is regulated in lymphocyte type and activation-state dependent manners. CD45RA is expressed on subsets of T and B cells rendering the CD45RA antibody particularly useful for studying subpopulations. For example, naïve T lymphocytes express CD45RA containing the A exon which is lost after activation and replaced by CD45R0. In this regard, the CD45RA antibody is a useful naïve T cells marker since they express CD45RA, and activated or memory T cells are CD45RA negative and CD45RO positive (Schlossman et al, 1995). CD45RA is differentially expressed on lymphomas and the CD45RA antibody may also be used to help identify or classify lymphomas such as the CD45RA positive B from the CD45R0 positive T cell lymphomas. Researchers are encouraged to consult the scientific literature for additional information about CD45RA expression, including the use of antibody panels that include a CD45RA antibody for characterizing a given malignancy. Applications: Suitable for use in FLISA, Western Blot, Immunofluorescence, Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-1ug/10e6 cells Western Blot: 0.5-1ug/ml Immunofluorescence: 1-2ug/ml Immunoprecipitation: 1-2ug/500ug protein lysate Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT Optimal dilutions to be determined by the researcher. Positive Control: Daudi, Raji, IM9, and human lymphocytes. Human lymph nodes and tonsils. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice