CD31 (PECAM-1, EndoCAM, PECA1, Platelet Endothelial Cell Adhesion Molecule 1, GPIIA)

Size

£422.00

SKU:: 138510
Purity:: Purified by Protein A/G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human CD31.
Clone:: C31.7
Format:: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138510-AF.
Extra Details:: CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 monoclonal antibody reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Flow Cytometry. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml Flow Cytometry: 1-2ug/10e6 cells Immunohistochemistry (FFPE): 1-2ug/ml/30min @ RT Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 9.0 for 45 min at 95°C, followed by cooling at RT for 20 minutes Optimal dilutions to be determined by the researcher. Positive Control: Tonsil, angiosarcoma Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice