138077
Lambda Light Chain (HRP)
Size
£1,259.00
- SKU:
- 138077
- Purity:
- Purified by affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Mouse lambda light chain
- Format:
- Supplied as a liquid in 50mM Hepes, pH 7.1, 0.1M sodium chloride, 0.2% BSA, 0.01% Thimerosal. Labeled with horseradish peroxidase (HRP).
- Extra Details:
- There are two types of light chain in mammals, the kappa chain; encoded by the immunoglobulin kappa locus on chromosome 2, and the lambda chain; encoded by the immunoglobulin lambda locus on chromosome 22. Antibodies are produced by B lymphocytes, each expressing only one class of light chain. Once set, light chain class remains fixed for the life of the B lymphocyte. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:10,000-1:100,000 Western Blot (colorimetric): 1:1000-1:10,000 Western Blot (chemiluminescent): 1:1000-1:30,000 Immunohistochemistry: 1:200-1:500 Immunocytochemistry: 1:200-1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice