137431
EIF1 (Eukaryotic Translation Initiation Factor 1)
Size
£626.00
- SKU:
- 137431
- Purity:
- Purified.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to residues near the N-terminal domain of human eIF1. Species Sequence Homology: canine
- Clone:
- 13G29(D7G3L)
- Format:
- Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
- Extra Details:
- Eukaryotic translation initiation factor 1 (eIF1) was first purified as a factor stimulating binding of Met-tRNA and mRNA to the ribosome (1,2). eIF1 is essential for growth in yeast and two classes of mutations in yeast eIF1 indicate a role for this protein in ensuring accurate translation initiation site selection (3). It has been demonstrated that eIF1 expression is stress-inducible, suggesting that modulation of translation initiation occurs during cellular stress (4). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice