USP5, Active, Recombinant, Human, FLAG-Tag (Ubiquitin Carboxyl-terminal Hydrolase 5, Ubiquitin Thiolesterase 5, Ubiquitin-specific Processing Protease 5, Deubiquitinating Enzyme 5, Isopeptidase T, ISOT)

Size

£1,002.00

SKU:: 137397
Molecular Weight:: 96
Purity:: Purified (~55%)
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 45mM Tris-HCl, pH 8.0, 124mM sodium chloride, 2.4mM potassium chloride, 10% glycerol, 3mM DTT, 90ug/ml FLAG peptide.
Extra Details:: USP5 cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. It’s involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. It binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition. Recombinant protein corresponding to aa2-858 from human USP5, fused to FLAG-tag at N-terminal, expressed in Sf9 insect cells via a baculovirus expression system. Molecular Weight: ~96kD Applications: Suitable for for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.