ROS1, Active, Recombinant, Human, GST-Tag (Proto-oncogene Tyrosine-protein Kinase ROS, Proto-oncogene c-Ros, Proto-oncogene c-Ros-1, Receptor Tyrosine Kinase c-ros Oncogene 1, c-Ros Receptor Tyrosine Kinase, MCF3, ROS)

Size

£925.00

SKU:: 137365
Molecular Weight:: 78
Purity:: Purified (~55%)
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM sodium chloride, 0.05% Tween 20, 3mM DTT, 20% glycerol.
Extra Details:: ROS1 is an orphan receptor tyrosine kinase (RTK) that plays a role in epithelial cell differentiation and regionalization of the proximal epididymal epithelium. May activate several downstream signaling pathways related to cell differentiation, proliferation, growth and survival including the PI3 kinase-mTOR signaling pathway. Mediates the phosphorylation of PTPN11, an activator of this pathway. May also phosphorylate and activate the transcription factor STAT3 to control anchorage-independent cell growth. Mediates the phosphorylation and the activation of VAV3, a guanine nucleotide exchange factor regulating cell morphology. May activate other downstream signaling proteins including AKT1, MAPK1, MAPK3, IRS1 and PLCG2. Source: Recombinant corresponding to aa1883-2347 from human ROS1, fused to GST-tag at N-terminal, expressed in Sf9 cells via a baculovirus expression system. Molecular Weight: ~78kD Applications: Suitable for use in the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Specific Activity: ~8pmol/min/ug Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Dry Ice