PDE4A Isoform 1, Active, Recombinant, Human, GST-Tag (cAMP-specific 3',5'-cyclic Phosphodiesterase 4A, DPDE2, PDE46, DPDE2)

Size

£1,028.00

SKU:: 137338
Molecular Weight:: 124
Purity:: Purified (~40%)
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM sodium chloride, 2.2mM potassium chloride, 4mM glutathione, 3mM DTT, 20% glycerol.
Extra Details:: Cyclic nucleotides are important second messengers that regulate and mediate a number of cellular responses to extracellular signals, such as hormones, light, and neurotransmitters. Cyclic nucleotide phosphodiesterases (PDEs) regulate the cellular concentrations of cyclic nucleotides and thereby play a role in signal transduction. PDE4A is a class IV cAMP-specific PDE (summary by Milatovich et al., 1994 [PubMed 8009369]). Source: Recombinant corresponding to aa2-end from human PDE4A4B, fused to GST-tag at N-terminal, expressed in a Baculovirus infected Sf9 cell expression system. Molecular Weight: ~124kD Applications: Suitable for use in the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Specific Activity: ~11610pmol/min/ug Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.