PDE1B, Active, Recombinant, Rat, His-GST Tag (Calcium/Calmodulin-dependent 3',5'-cyclic Nucleotide Phosphodiesterase 1B, Cam-PDE 1B, 63kD Cam-PDE, Pde1b1)

Size

£1,009.00

SKU:: 137328
Molecular Weight:: 89
Purity:: Purified (~70%)
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM sodium chloride, 2.2mM potassium chloride, 200mM Imidazole, 3mM DTT, 20% glycerol.
Extra Details:: PDE1B is a cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a preference for cGMP as a substrate. Source: Recombinant corresponding to full length rat PDE1B, fused to His-tag at N-terminal, expressed in Sf9 insect cells via a baculovirus expression system. Molecular Weight: ~89kD Specific Activity: ~3600pmol/min/ug Unit Definition: One unit is defined as the amount of enzyme that will convert 1pmole of 3′, 5′-cAMP to 5′-AMP per min at 37°C. Applications: Suitable for use in the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.