Glutathione S-Transferase, Epitope Tag (GST-Tag)

Size

£576.00

SKU:: 137274
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant corresponding to Glutathione-S-transferase protein (S. japonicum).
Clone:: 13G6
Format:: Supplied as a liquid in 10mM PBS, pH 7.2, 0.05% sodium azide.
Extra Details:: Human glutathione S-transferases (GSTs) are a large multigene family of isoenzymes, which catalyse the conjugation of glutathione to electrophilic substrates. These enzymes are involved in the detoxification of both endogenous and exogenous electrophiles, which can react with cellular components such as DNA. The modification of DNA by reactive compounds can initiate carcinogenesis and the GSTs are believed to play a role in carcer prevention by inactivating carcinogens. There are four major structural classes of mammalian cytosolic GSTs (alpha, mu, pi and theta). Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Dot Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:3000 Immunocytochemistry: 1:500-1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.