GCLM (Glutamate-cysteine Ligase, Modifier Subunit, Gamma-glutamylcysteine Synthetase)

Size

£406.00

SKU:: 137065
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human GCLM, expressed in E.coli.
Clone:: 16C50
Format:: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Extra Details:: GCLM (Glutamate-cysteine ligase, modifier subunit), also known as gamma-glutamylcysteine synthetase, is first rate limiting enzyme of glutathione synthesis. GCLM is heterodimer of a heavy catalytic subunit and a light regulatory subunit that is responsive to inflammation, phenolic antioxidants, heat shock, oxidants and cytokines. GCLM deficiency has been implicated in some forms of hemolytic anemia. Also, GCLM is a molecular target for amelioration of cisplatin restance in lung cancer. Applications: Suitable for use in ELISA, Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:3000. Optimal dilutions to be determined by the researcher. Hybridoma: Mouse F0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.