TCF4/TCF7L2 (Transcription Factor 4, TCF-4, Class B Basic Helix-loop-helix Protein 19, Immunoglobulin Transcription Factor 2, ITF-2, SEF-2, SL3-3 Enhancer Factor 2, ITF2, SEF2)

Size

£626.00

SKU:: 136969
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to residues surrounding Leu331 of human TCF4/TCF7L2. Species sequence homology: mouse and chicken
Clone:: C48H11
Format:: Supplied as a liquid in 10mM HEPES, p.H 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Extra Details:: LEF1 and TCF are members of the high mobility group (HMG) DNA binding protein family of transcription factors which consists of the following: Lymphoid enhancer factor 1 (LEF1), T Cell Factor 1 (TCF1), TCF3 and TCF4. LEF1 and TCF1 were originally identified as important factors regulating early lymphoid development and act downstream in Wnt signaling. LEF1/TCF bind to Wnt response elements to provide a docking site for b-catenin, which translocates to the nucleus to promote the transcription of target genes upon activation of Wnt signaling. LEF1/TCF proteins are dynamically expressed during development and aberrant activation of the Wnt signaling pathway is involved in many types of cancers including colon cancer. TCF4, also known as TCF7L2, is expressed widely during development. Gene targeting studies indicate that TCF4 is required to maintain the crypt stem cells of the small intestine. TCF4 has several splicing isoforms that are expressed differentially in tissues and during cancer progression. Studies also indicate that a variant of the TCF4 gene confers an increased risk of type 2 diabetes. Applications: Suitable for use in Western Blot, ChIP and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Immunoprecipitation: 1:50 ChIP: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice