Casein Kinase 1 alpha (CK1a)

Size

£576.00

SKU:: 136871
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Chicken/Avian
Immunogen:: Recombinant proteiin corresponding to full length human CK1a (shortest isoform)
Format:: Supplied as a liquid in PBS, 0.02% sodium azide
Extra Details:: Many serine threonine (ser/thr) protein kinases are activated by specific small signalling molecules such as calcium, cAMP, cGMP while others appear to be cofactor independent. The casein kinases belong to this group, and how their activity is regulated is still something of a mystery (1,2). There are two broad families of these kinases called CKI and CKII (or CK1 and CK2). They were originally isolated since extracts of most cells contain two distinct fractions of kinase activity both of which are able to phosphorylate the milk protein casein. It is now known that the CKI and CKII activities represent two different families of distinct kinases, each consisting of several different gene products. The different proteins clearly represent an ancient and unusually conserved family of kinases, sharing some unique sequence characteristics not found in other protein kinases. The CKI family consists of several different genes, divided in α, β, γ, δ and ε (alpha, beta, gamma, delta and epsilon), some of which are encoded by more than one gene. The single mammalian CK1α gene can generate four different proteins by altering transcription. The CK1 alpha proteins are involved in the control of protein degradation, and phosphorylate acidic regions of their substrate molecules. Typically the target Ser/Thr residues closely C-terminal to two or three Asp or Glu acid residues. Alternately, phosphorylation on residues 3 or 4 amino acids N-terminal to a Ser/Thr residue can also favor CKI phosphorylation. This means that CKI family enzymes often phosphorylate one or more Ser/Thr residues C-terminal to a phosphorylation site generated by the activity of another protein kinase. This antibody was made against a recombinant construct based on the smallest CKIα isotype and will therefore bind to all CKIα isotypes. Applications: Suitable for use in Immunofluorescence, Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence: 1:500-1:1000 Western Blot: 1:10,000 Immunocytochemistry: 1:5000 (ABC or other enzyme linked immunocytochemical procedures) Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice