FIH (Factor inhibiting HIF1 (Hypoxia-inducible Factor), Hypoxia-inducible factor 1-alpha Inhibitor, Hypoxia-inducible Factor Asparagine Hydroxylase)

Size

£626.00

SKU:: 136778
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to full length BL21 (DE3) cell expressed in E. coli.
Clone:: FIH 162c
Format:: Supplied as a liquid in PBS, pH 7.4, 50% glycerol.
Extra Details:: FIH, Factor inhibiting HIF1 (hypoxia-inducible factor), is an asparaginyl hydroxylase. FIH in conjunction with VHL represses HIF-1 transcriptional activity by disrupting the interaction of HIF-1 with the transcriptional coactivators CBP/p300, and by recruiting histone deacetylases. FIH activity is inhibited during hypoxia (1-3). Recent studies show that low nuclear expression of FIH is a strong independent prognostic factor for a poor overall survival in clear cell renal cell cancinoma (4). Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 10ug/ml Immunohistochemistry: Paraffin Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.