136519
VEGFR-3 (Tyrosine-protein kinase receptor FLT4, VEGFR3, FEGF)
Size
£544.00
- SKU:
- 136519
- Application:
- WB
- Purity:
- Purified by affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- VEGFR-3-Fc fusion protein
- Clone:
- AFL4
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
- Extra Details:
- 136519 detects multiple bands by Western Blot: a ~140kD band corresponding to cleaved/soluble VEGFR-3, and 220 and 230kD bands corresponding to unglycosylated and glycosylated full length VEGFR-3 (Bando, et al. 2004. Int J Canc. 111:184.) FLT4, a VEGF Receptor type protein kinase, is an endothelial cell-specific receptor. Binding of the extracellular domain of FLT4 to the vascular endothelial growth factor-related protein VEGF-C stimulates tyrosine phosphorylation and mitogenesis of endothelial cells. FLT4 (-/-) mice have been shown to have defective blood vessel development in early embryos and to experience cardiovascular failure at embryonic day 9.5. A mutation in the FLT4 gene has been implicated in human hereditary lymphedema type I. Similarly, a mutation in the conserved catalytic domain of FLT4, in close proximity to the FLT4 mutations in human primary lymphedema, has been linked to the Chy mouse mutant. Applications: Suitable for use in Western Blot. Though not tested this antibody has been reported by to work for immunohistochemical staining of frozen tissue sections. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice