136361
Prostaglandin E Synthase (Cytosolic)
Size
£731.00
- SKU:
- 136361
- Application:
- WB
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to an internal region of human protein cPGES synthase
- Format:
- Supplied as a lyophilized powder in TBS, pH 7.4. Reconstitute with 500ul sterile ddH2O.
- Extra Details:
- Prostaglandin E synthase (PGES) catalyzes the isomerization of PGH2 to PGE2. Cytosolic and microsomal PGES enzymes have been cloned and characterized. Cytosolic PGES (cPGES) is a glutathione-dependent enzyme with a predicted size of 18.6kD (23kD on SDS-PAGE). The enzyme is expressed in a wide variety of tissues and cells, the levels of which are unaffected by treatment with IL-1β and TNF-α.1 However, enzyme expression increases approximately 3-fold in rat brain following LPS treatment.1 Microsomal PGES (mPGES) is a 16kD protein expressed in a variety of tissues including prostate, testes, and small intestine, as well as in A549 and HeLa cells.2 In contrast to cPGES, mPGES protein expression is increased in A549 cells following treatment with IL-1β.2 The two enzymes show <10% homology at the amino acid level. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice