Pak1 (Alpha-PAK, CDC42/RAC effector kinase PAK-A, p21-activated kinase 1, p65-PAK)

Size

£709.00

SKU:: 136311
Purity:: Purified by immunoaffinity chromatography
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to human PAK1.
Format:: Supplied as a liquid in PBS, (without Mg2+, Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Extra Details:: PAK1, a PAK (STE20) type protein kinase, is an effector for the small GTP binding proteins Cdc42 and Rac in the regulation of cell motility and morphology. The activated protein has been shown to act on a variety of targets, including actin-binding protein filamin A, myosin light chain kinase, histone H3 and the stress-activated protein kinase JNK1. The PAK1 gene has been mapped to chromosome 11q13, a region of amplification in a subset of estrogen receptor positive breast carcinomas prone to metastasis. Applications: Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence: 1:100-1:500 ELISA: 1:1000 Western Blot: 1:500-1:1000 Immunohistochemistry (Paraffin): 10ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.