GUCY1A1 (Guanylate Cyclase alpha Subunit (Soluble))

Size

£539.00

SKU:: 136137
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to the internal region of human GUCY1A1.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: Soluble guanylate cyclase is a heterodimeric enzyme, composed of α and β subunits, that synthesizes cGMP from GTP. The enzyme is activated by the binding of nitric oxide or carbon monoxide to the heme group of the enzyme. Chronic hypoxia upregulates soluble guanylate expression in rat lung. The α1 subunit contains 690-717 amino acids and has a molecular mass of 77-82kD. The cloned β1 subunit of guanylate cyclase from human, bovine, and rat sources contains 619 amino acids and has a molecular mass of approximately 70,000. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:20 Immunohistochemistry (Paraffin): 1:80 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.