Cyclin E (CCNE, Cyclin E1, G1/S-specific Cyclin-E1, CCNE1)

Size

£735.00

SKU:: 136019
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to human CCNE1 at C-Terminal.
Format:: Supplied as a liquid in PBS, (without Mg2+, Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Extra Details:: Cyclin E activity is mediated via its activation of cyclin dependent kinase 2 protein and is modulated by certain tumor suppressor proteins. Cyclin E overexpression shortens the length of G1 phase, accelerating progres- sion of the cell cycle into S phase. This antibody is specific for cyclin E and may help characterize its expression in a variety of tissue types. Applications: Suitable for use in Immunofluorescene, ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunofluorescene: 1:500-1:1000 ELISA: 1:10,000 Western Blot: 1:500-1:1000 Immunohistochemistry (Paraffin): 10ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.