135946
Caspase-9 (Caspase 9, ICE-LAP6, Mch6, Apaf 3)
Size
£735.00
- SKU:
- 135946
- Purity:
- Purified by immunoaffinity chromatography
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to human Caspase 9.
- Format:
- Supplied as a liquid in PBS, (without Mg2+, Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- Caspase 9 is a cysteine C14 endopeptidase that mediates oncogene and drug-induced apoptosis. It is activated upon binding to Apaf-1/cytochrome c and inhibited upon phosphorylation by the serine/ threonine kinase Akt. Activated caspase-9 cleaves downstream caspases such as Caspase-3, -6 and -7 initiating the caspase cascade. Like other caspases, Caspase-9 plays a critical role in mediating apoptosis during normal brain development. Caspase-9 also cleaves amyloid beta-protein precursors, principal constituent of senile plaques, and is involved in neuronal death observed in Alzheimer disease. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:10,000 Western Blot: 1:500-1:1000 Immunohistochemistry (Paraffin): 10ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice