133882-HRP

SSX2 (Synovial Sarcoma X Breakpoint 2, Cancer/testis Antigen 5.2, CT5.2, CT5.2a, Protein SSX2, SSX2A, SSX2B, Tumor Antigen HOM-MEL-40, MGC119055, MGC15364, MGC3884) (HRP)

Size

£914.00

SKU:: 133882-HRP
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Full-length recombinant corresponding to aa1-224 from human SSX2 (AAH02818) with GST tag. MW of the GST tag alone is 26kD.
Clone:: 1A4
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Extra Details:: Synovial sarcoma, X breakpoint 2, also known as SSX2, is a human gene. The product of this gene belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. SSX1, SSX2 nd SSX4 genes have been involved in the t (X;18) translocation characteristically found in all synovial sarcomas. The SSX1 and SSX2 genes encode closely related proteins (81% identity) of 188aa that are rich in charged amino acids. The N-terminal portion of each SSX protein exhibits homology to the Kruppel-associated box (KRAB), a transcriptional repressor domain previously found nly in Kruppel-type zinc finger proteins. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 1.5ug/ml Optimal dilutions to be determined by the researcher. AA Sequence: MNGDDAFARRPTVGAQIPEKIQKAFDDIAKYFSKEEWEKMKASEKIFYVYMKRKYEAMTKLGFKATLPPFMCNKRAEDFQGNDLDNDPNRGNQVERPQMTFGRLQGISPKIMPKKPAEEGNDSEEVPEASGPQNDGKELCPPGKPTTSEKIHERSGNREAQEKEERRGTAHRWSSQNTHNIGRFSLSTSMGAVHGTPKTITHNRDPKGGNMPGPTDCVRENSW* Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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