RAD17 (Cell Cycle Checkpoint Protein RAD17, hRad17, RF-C/Activator 1 Homolog, R24L, FLJ41520)

Size

£685.00

SKU:: 132254
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Partial recombinant corresponding to aa1-110, from human RAD17 (AAH32304) with GST tag. MW of the GST tag alone is 26kD.
Clone:: 2G12
Format:: Supplied as a liquid in PBS, pH 7.2.
Extra Details:: Rad17 is an essential component of cell cycle checkpoints activated by DNA damage and disruption of DNA replication. DNA lesions such as single- and double-strand breaks, inter- and intra-strand cross-links, and base modifications hamper transcription and replication, resulting in cell cycle arrest, apoptosis, and mutagenesis. Phosphorylation of Rad17 at Ser635 and Ser645 is a critical early event during checkpoint signaling in DNA damaged cells. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. AA Sequence: MNQVTDWVDPSFDDFLECSGVSTITATSLGVNNSSHRRKNGPSTLESSRFPARKRGNLSSLEQIYGLENSKEYLSENEPWVDKYKPETQHELAVHKKKIEEVETWLKAQV Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.