Phosphodiesterase 5A (PDE5A, cGMP-Specific 3',5'-Cyclic Phosphodiesterase, cGMP-Binding cGMP-Specific Phosphodiesterase, CGB-PDE, PDE5)

Size

£685.00

SKU:: 131278
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Partial recombinant protein corresponding to aa29-138 from human Phosphodiesterase 5A with GST tag. MW of the GST tag alone is 26kD.
Clone:: 9H5
Format:: Supplied as a liquid in PBS, pH 7.4.
Extra Details:: Phosphodiesterase 5A, a cGMP-specific phosphodiesterase, has been implicated in primary pulmonary hypertension, cardiac failure and erectile dysfunction. PDE5A also may function in the regulation of coronary blood flow. PDE5A is the predominant enzyme responsible for cGMP hydrolysis in trabecular smooth muscle. PDE5A activitity is inhibited by sildenafil (Viagra) and zaprinast. At least three alternatively spliced transcripts have been reported. Applications: Suitable for use in ELISA, Western Blot and Immunofluorescence. Other applications have not been tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. AA Sequence: SVEAWLDDHWDFTFSYFVRKATREMVNAWFAERVHTIPVCKEGIRGHTESCSCPLQQSPRADNSVPGTPTRKISASEFDRPLRPIVVKDSEGTVSFLSDSEKKEQMPLTP* Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.