NQO1 (NAD(P)H Dehydrogenase [Quinone] 1, Azoreductase, DT-diaphorase, DTD, QR1, Menadione Reductase, NAD(P)H:quinone Oxidoreductase 1, Phylloquinone Reductase, Quinone Reductase 1, DIA4, NMOR1)

Size

£685.00

SKU:: 130520
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Full length recombinant corresponding to aa1-274 from human NQO1 (AAH07659) with GST tag. MW of the GST tag alone is 26kD.
Clone:: 1E3-A6
Format:: Supplied as a liquid in PBS, pH 7.2.
Extra Details:: NQO1 is a 274aa protein belonging to the NAD(P)H dehydrogenase (quinone) family. Its enzymatic activity prevents reduction of quinones that results in the production of radical species and is involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. It plays a role in regulating ubiquitin-independent degradation of ornithine decarboxylase by the 20S proteasome and regulates p53 proteasomal degradation in cancer. Defects in NQO1 lead to tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, susceptibility to various forms of cancer and Alzheimer's disease (AD). It is widely expressed. Applications: Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Immunofluorescence: 10ug/ml Sandwich ELISA: The detection limit is ~0.3ng/ml as a capture antibody Optimal dilutions to be determined by the researcher. Amino Acid Sequence: MVGRRALIVLAHSERTSFNYAMKEAAAAALKKKGWEVVESDLYAMNFNPIISRKDITGKLKDPANFQYPAESVLAYKEGHLSPDIVAEQKKLEAADLVIFQFPLQWFGVPAILKGWFERVFIGEFAYTYAAMYDKGPFRSKKAVLSITTGGSGSMYSLQGIHGDMNVILWPIQSGILHFCGFQVLEPQLTYSIGHTPADARIQILEGWKKRLENIWDETPLYFAPSSLFDLNFQAGFLMKKEVQDEEKNKKFGLSVGHHLGKSIPTDNQIKARK Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice