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130251-BIOTIN

Nebulin (NEB, DKFZp686C1456, FLJ39568, FLJ39584, NEB177D, NEM2) (Biotin)

Size

£914.00

SKU:
130251-BIOTIN
Purity:
Purified by Protein A affinity chromatography.
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Mouse
Immunogen:
Partial recombinant corresponding to aa2108-2217 from human Nebulin with GST tag. MW of the GST tag alone is 26kD.
Clone:
1F4
Format:
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Extra Details:
Nebulin is a giant protein component of the cytoskeletal matrix that coexists with the thick and thin filaments within the sarcomeres of skeletal muscle. In most vertebrates, nebulin accounts for 3 to 4% of the total myofibrillar protein and its size varies from 600 to 800kD in a manner that is tissue-, species-,and developmental stage-specific. The nebulin gene contains 183 exons in a 249kb genomic region. Nebulin is mapped to chromosome 2. Nebulin is a giant filamentous protein specific for vertebrate skeletal muscles. Mutations in the nebulin gene associated with autosomal recessive nemaline myopathy. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. AA Sequence: NTKTSYHTPADMLSVTAAKDAQANITNTNYKHLIHKYILLPDAMNIELTRNMNRIQSDNEYKQDYNEWYKGLGWSPAGSLEVEKAKKATEYASDQKYRQHPSNFQFKKLT Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.  For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.  Note: Applications are based on unconjugated antibody.
Shipping Conditions:
Blue Ice