129568-HRP
MEKK1 (MAPK/ERK Kinase Kinase 1, MEK Kinase 1, MEKK 1, MEKK1, MEKK, Mitogen-activated Protein Kinase Kinase Kinase 1, MAP3K1, MAPKKK1, SRXY6) (HRP)
Size
£914.00
- SKU:
- 129568-HRP
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Partial recombinant corresponding to aa1211-1310 from human MAP3K1 (XP_042066) with GST tag. MW of the GST tag alone is 26kD.
- Clone:
- 2F6
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
- Extra Details:
- MEKK1, a MAP3K type protein kinase, is a member of the stress-activated protein kinase (SAPK) pathways which convey pro-apoptotic signals. The protein consists of a C-terminal protein kinase domain and an extensive N-terminal domain that includes two proline-rich segments containing putative binding sites for proteins with SH3 domains, a consensus binding site for 14-3-3 proteins, two PH domains and an acid rich motif with two sites for cleavage by proapoptotic caspase cysteine proteases. MEKK1 activates MKK4/MAP2K4 and, to a lesser extent, MKK7/MAP2K7 in the SAPK cascade. MEKK1 also has been shown to activate the transcription factor CCAAT/enhancer-binding protein-beta-dependent gene expression via MEK1-ERK1/2 kinases in response to IFN-gamma. In MEKK1 (-/-) mouse embryonic stem cells the response of JNK to microtubule disruption, cold stress, hyperosmolarity and serum factors is lost or altered, and the response of ERK to hyperosmolarity and serum factors is diminished. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. AA Sequence: SKNSMTLDLNSSSKCDDSFGCSSNSSNAVIPSDETVFTPVEEKCRLDVNTELNSSIEDLLEASMPSSDTTVTFKSEVAVLSPEKAENDDTYKDDVNHNQK Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice