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129259-HRP

STRADA (LYK5, STE20-related Kinase Adapter Protein alpha, STRAD alpha, STE20-related Adapter Protein, Serologically Defined Breast Cancer Antigen NY-BR-96, STRAD, FLJ90524) (HRP)

Size

£914.00

SKU:
129259-HRP
Purity:
Purified by Protein A affinity chromatography.
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Mouse
Immunogen:
Partial recombinant corresponding to aa251-347 from human LYK5 (NP_699166) with GST tag. MW of the GST tag alone is 26kD.
Clone:
4E4
Format:
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Extra Details:
Pseudokinase which, in complex with CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta), binds to and activates STK11/LKB1. Adopts a closed conformation typical of active protein kinases and binds STK11/LKB1 as a pseudosubstrate, promoting conformational change of STK11/LKB1 in an active conformation. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 6ug/ml Optimal dilutions to be determined by the researcher. AA Sequence: LLEKLNGTVPCLLDTSTIPAEELTMSPSRSVANSGLSDSLTTSTPRPSNGDSPSHPYHRTFSPHFHHFVEQCLQRNPDARYPCWPGPGLRESRGCS Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
Shipping Conditions:
Blue Ice