GRB2 (Growth Factor Receptor-bound Protein 2, Adapter Protein GRB2, Protein Ash, SH2/SH3 Adapter GRB2, ASH)

Size

£698.00

SKU:: 127529
Purity:: Ascites
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Full length recombinant corresponding to aa1-217 from human GRB2 (AAH00631) with GST tag. MW of the GST tag alone is 26kD.
Clone:: 4C6-H6
Format:: Supplied as a liquid in ascites fluid.
Extra Details:: Grb2 is an adaptor protein which has no enzymatic activity; however, Grb2 plays an important role in signaling cascades by physically linking other proteins through its phosphotyrosine-binding SH2 domain and its proline-binding SH3 domain. Grb2 associates with the guanine nucleotide exchange factor Sos via its SH3 domain. In response to cell stimulation with growth factors, growth factor receptors are autophosphorylated on cytoplasmic tyrosine residues. Grb2's association with these tyrosine residues via its SH2 domain permits the localization of Sos with the growth factor receptor near the cell membrane. Sos is then able to activate the membrane associated protein Ras which in turn activates the MAPK cascade and influences gene expression. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. AA Sequence: MEAIAKYDFKATADDELSFKRGDILKVLNEECDQNWYKAELNGKDGFIPKNYIEMKPHPWFFGKIPRAKAEEMLSKQRHDGAFLIRESESAPGDFSLSVKFGNDVQHFKVLRDGAGKYFLWVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQVPQQPTYVQALFDFDPQEDGELGFRRGDFIHVMDNSDPNWWKGACHGQTGMFPRNYVTPVNRNV Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice