127043-ML490
FYN (Tyrosine-protein Kinase Fyn, Proto-oncogene Syn, Proto-oncogene c-Fyn, Src-like Kinase, SLK, p59-Fyn) (MaxLight 490)
Size
£914.00
- SKU:
- 127043-ML490
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Full length recombinant corresponding to aa1-482 from human FYN (AAH32496) with GST tag. MW of the GST tag alone is 26kD.
- Clone:
- 3G11-F9
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
- Extra Details:
- MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. Fyn is a member of the Src family of protein tyrosine kinases. Fyn has been shown to be involved in T cell and B cell activation as well as keratinocyte differentiation. Fyn can regulate the activation of PI-3 kinase in CTLL-2 cells, a murine interleukin 2 responsive cell line. In T cells, Fyn associates with the T cell antigen receptor and Thy-1. A study of human T-cells revealed that Lck and Fyn had different intracellular locations, suggesting that these two members of the Src family regulate different aspects of T cell activation. The Src homology 3 (SH3) region of Fyn can bind the proline-rich domain of the Fas ligand. In addition, Raf-1 may form a complex with Fyn through its Src Homology 2 (SH2) domain and serine phosphorylation of Raf-1. Applications: Suitable for use in FLISA and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. AA Sequence: MGCVQCKDKEATKLTEERDGSLNQSSGYRYGTDPTPQHYPSFGVTSIPNYNNFHAAGGQGLTVFGGVNSSSHTGTLRTRGGTGVTLFVALYDYEARTEDDLSFHKGEKFQILNSSEGDWWEARSLTTGETGYIPSNYVAPVDSIQAEEWYFGKLGRKDAERQLLSFGNPRGTFLIRESETTKGAYSLSIRDWDDMKGDHVKHYKIRKLDNGGYYITTRAQFETLQQLVQHYSGTWNGNTKVAIKTLKPGTMSPESFLEEAQIMKKLKHDKLVQLYAVVSEEPIYIVTEYMNKGSLLDFLKDGEGRALKLPNLVDMAAQVAAGMAYIERMNYIHRDLRSANILVGNGLICKIADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELVTKGRVPYPGMNNREVLEQVERGYRMPCPQDCPISLHELMIHCWKKDPEERPTFEYLQSFLEDYFTATEPQYQPGENL Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice