126259-ML490
ELA1 (Chymotrypsin-like Elastase Family Member 1, Elastase-1, Pancreatic Elastase 1, CELA1) (MaxLight 490)
Size
£914.00
- SKU:
- 126259-ML490
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Partial recombinant corresponding to aa159-257 from human ELA1 (NP_001962) with GST tag. MW of the GST tag alone is 26kD.
- Clone:
- 4H5
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
- Extra Details:
- MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. Human Elongin A (EloA), otherwise known as transcription elongation factor B polypeptide 3, a general transcription elongation factor, stimulates RNA polymerase II transcription elongation beyond template-encoded arresting sites. Elongin exists as a heterotrimer consisting of an A (A1, A2 or A3), B and C subunit. Subunit A is transcriptionally active, with activity strongly enhanced by binding to the dimeric complex of the elongin regulatory subunits B and C (elongin BC complex), which is mediated by subunit C. Applications: Suitable for use in FLISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml Optimal dilutions to be determined by the researcher. AA Sequence: LAQTLQQAYLPSVDYAICSSSSYWGSTVKNTMVCAGGDGVRSGCQGDSGGPLHCLVNGKYSVHGVTSFVSSRGCNVSRKPTVFTQVSAYISWINNVIAS Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice