DCIR (C-type Lectin Domain Family 4, Member A, C-type Lectin Superfamily Member 6, Dendritic Cell Immunoreceptor, Clec4a2, Clecsf6, Dcir, CLEC4A)

Size

£685.00

SKU:: 125663
Application:: WB
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Full length human CLEC4A, aa1-237 (NP_057268.1).
Format:: Supplied as a liquid in PBS, pH 7.2.
Extra Details:: A member of the calcium-dependent (C-type) lectin receptor (CLR) family, DCIR (for dendritic cell (DC) immunoreceptor), is a type II membrane glycoprotein of 237aa with a single carbohydrate recognition domain (CRD), closest in homology to those of the macrophage lectin and hepatic asialoglycoprotein receptors. The intracellular domain of DCIR contains a consensus immunoreceptor tyrosine-based inhibitory motif (ITIM). A mouse cDNA, encoding a homologous protein has been identified. Northern blot analysis shows that DCIR mRNA is predominantly transcribed in hematopoietic tissues. DCIR displays a typical lectin CRD and is expressed on DCs, monocytes, macrophages, B lymphocytes, and granulocytes, but not detected on NK and T cells. DCIR is strongly expressed by DCs derived from blood monocytes cultured with GM-CSF and IL-4. DCIR is preferentially expressed by monocyte-related rather than Langerhans cell related DCs obtained from CD34+ progenitor cells. Finally, DCIR expression is down-regulated by signals inducing DC maturation such as CD40 ligand, LPS, or TNF-alpha. Thus, DCIR is differentially expressed on DCs depending on their origin and stage of maturation:activation. DCIR represents a novel surface molecule expressed by antigen-presenting cells, and of potential importance in regulation of DC function. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. AA Sequence: MTSEITYAEVRFKNEFKSSGINTASSAASKERTAPHKSNTGFPKLLCASLLIFFLLLAISFFIAFVIFFQKYSQLLEKKTTKELVHTTLECVKKNMPVEETAWSCCPKNWKSFSSNCYFISTESASWQDSEKDCARMEAHLLVINTQEEQDFIFQNLQEESAYFVGLSDPEGQRHWQWVDQTPYNESSTFWHPREPSDPNERCVVLNFRKSPKRWGWNDVNCLGPQRSVCEMMKIHL Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice