125532
Cyclin B1 (G2/Mitotic-specific Cyclin-B1, CCNB1, CCNB)
Size
£760.00
- SKU:
- 125532
- Application:
- WB
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Full length human CCNB1, aa1-433 (NP_114172.1).
- Format:
- Supplied as a liquid in PBS, pH 7.2.
- Extra Details:
- Cyclins were originally discovered as proteins whose accumulation during the interphase and destruction at mitosis played a critical role in the progression through the eukaryotic cell cycle. It was subsequently found that cyclins control the cell cycle by binding to Ser/Thr cyclin-dependent kinases (CDKs) and regulating their activities. Human cyclins have been grouped into 5 types, A through E, based largely upon sequence similarity. Cyclin B is essential for the control of the cell cycle at the G2/M (mitosis) transition. The expression of cyclin B1 is cell-cycle regulated, peaking at the G2/M transition. It interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF), and thus the complex regulates the progression of cells through G2 into M phase. It maps to 5q12 region of human chromosome. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. AA Sequence: MALRVTRNSKINAENKAKINMAGAKRVPTAPAATSKPGLRPRTALGDIGNKVSEQLQAKMPMKKEAKPSATGKVIDKKLPKPLEKVPMLVPVPVSEPVPEPEPEPEPEPVKEEKLSPEPILVDTASPSPMETSGCAPAEEDLCQAFSDVILAVNDVDAEDGADPNLCSEYVKDIYAYLRQLEEEQAVRPKYLLGREVTGNMRAILIDWLVQVQMKFRLLQETMYMTVSIIDRFMQNNCVPKKMLQLVGVTAMFIASKYEEMYPPEIGDFAFVTDNTYTKHQIRQMEMKILRALNFGLGRPLPLHFLRRASKIGEVDVEQHTLAKYLMELTMLDYDMVHFPPSQIAAGAFCLALKILDNGEWTPTLQHYLSYTEESLLPVMQHLAKNVVMVNQGLTKHMTVKNKYATSKHAKISTLPQLNSALVQDLAKAVAKV Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice