125237-BIOTIN
CORO1A (Coronin-1A, Coronin-like Protein A, Clipin-A, Coronin-like Protein p57, Tryptophan Aspartate-containing Coat Protein, TACO, CORO1, FLJ41407, MGC117380) (Biotin)
Size
£927.00
- SKU:
- 125237-BIOTIN
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Partial recombinant corresponding to aa360-462 from CORO1A (NP_009005) with GST tag. MW of the GST tag alone is 26kD.
- Clone:
- 4G10
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
- Extra Details:
- Coronin 1a belongs to the WD repeat coronin family and contains 5 WD repeats. It may be an essential component of the cytoskeleton of highly motile cells, with roles both in the invagination of large pieces of plasma membrane, as well as in forming protrusions of the plasma membrane involved in cell locomotion. In cell infected by mycobacteria, its retention on the phagosomal membrane prevents fusion between phagosomes and lysosomes. In the CNS, coronin 1A is expressed exclusively by microglial cells. Although its function is not entirely clear, it may act as a linkage protein between the actin cytoskeleton and the plasma membrane, and perhaps the extracellular matrix. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml Optimal dilutions to be determined by the researcher. AA Sequence: QEDLYPPTAGPDPALTAEEWLGGRDAGPLLISLKDGYVPPKSRELRVNRGLDTGRRRAAPEASGTPSSDAVSRLEEEMRKLQATVQELQKRLDRLEETVQAK* Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice