125200-HRP

Collapsin Response Mediator Protein 4 (CRMP-4, CRMP4, Dihydropyrimidinase-like 3, DPYSL3, Dihydropyrimidinase-related Protein 3, DRP-3, DRP3, LCRMP, TUC4, Unc-33-like Phosphoprotein, ULIP, Ulip-1) (HRP)

Size

£914.00

SKU:: 125200-HRP
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Partial recombinant corresponding to aa457-555 from human DPYSL3 (NP_001378) with GST tag. MW of the GST tag alone is 26kD.
Clone:: 1B8
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Extra Details:: DRP-3 belongs to the hydantoinase/dihydropyrimidase subfamily. It is required for signalling by class 3 semaphorins and remodelling of the cytoskeleton. It also plays a role in axon guidance, neuronal growth cone collapse and cell migration. It exists as a homotetramer, and heterotetramer with CRMP1, DPYSL2, DPYSL4 or DPYSL5. The unprocessed precursor is 570 amino acids long and has an estimated molecular weight of 61.9kD. It is mainly expressed in heart and skeletal muscle, but is also strongly expressed in fetal brain and spinal cord. It localizes to the cytoplasm and growth cone. This protein interacts with synaptic vesicle protein 2 and SH3A domain of intersectin. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml Optimal dilutions to be determined by the researcher. AA Sequence: GNLHVTQGAGRFIPCSPFSDYVYKRIKARRKMADLHAVPRGMYDGPVFDLTTTPKGGTPAGSARGSPTRPNPPVRNLHQSGFSLSGTQVDEGVRSASKR Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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