124997-ML550
CIB1 (Calcium and Integrin-binding Protein 1, CIB, Calcium- and Integrin-binding Protein, CIBP, Calmyrin, DNA-PKcs-interacting Protein, Kinase-interacting Protein, KIP, SNK-interacting Protein 2-28, SIP2-28, CIB, KIP, PRKDCIP) (MaxLight 550)
Size
£914.00
- SKU:
- 124997-ML550
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Full length recombinant corresponding to aa1-191 from CIB1 (AAH00846) with GST tag. MW of the GST tag alone is 26kD.
- Clone:
- 4D2-3A8
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
- Extra Details:
- MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000. CIB1 (Calcium and Integrin Binding protein1; also calmyrin, KIP and CIBP) is a 22-23kD, Ca++ binding member of the CIB family of proteins. It is widely expressed, being found in neurons, platelets/megakaryocytes, skeletal muscle myocytes and lymphocytes. CIB1 is associated with the cytosolic side of the plasma membrane, and has multiple binding partners, including InsP3R, GPIIb aIIb, presenilin 2, and NBR1 plus FEZ. When CIB1 binds InsP3R, this ER-embedded receptor is both activated, and later desensitized to subsequent ligand binding. Relative to GPIIb, CIB1 interaction with the aIIbB 3 integrin on platelets following thrombin exposure appears to inhibit integrin activation, thus providing a tight control on subsequent platelet binding to fibrinogen. Human CIB1 is 191aa in length. It contains a utilized myristoylation site at 2aa, followed by two EF-hand domains (aa103-183). CIB1 is suggested to act as a monomer. There is at least one potential isoform variant that shows a 40aa insertion after 29aa. Full length human CIB1 (aa1-191) shares 94% aa sequence identity with mouse CIB1. Applications: Suitable for use in FLISA, Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence: 10ug/ml Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml Optimal dilutions to be determined by the researcher. AA Sequence: MGGSGSRLSKELLAEYQDLTFLTKQEILLAHRRFCELLPQEQRSVESSLRAQVPFEQILSLPELKANPFKERICRVFSTSPAKDSLSFEDFLDLLSVFSDTATPDIKSHYAFRIFDFDDDGTLNREDLSRLVNCLTGEGEDTRLSASEMKQLIDNILEESDIDRDGTINLSEFQHVISRSPDFASSFKIVL Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice