ANXA1 (Annexin A1, Annexin I, Annexin-1, Calpactin II, Calpactin-2, Chromobindin-9, Lipocortin I, Phospholipase A2 Inhibitory Protein, p35, ANX1, LPC1) (MaxLight 650)

Size

£934.00

SKU:: 123350-ML650
Application:: WB
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Full length human ANXA1, aa1-346 (NP_000691.1).
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
Extra Details:: MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. The annexin superfamily consists of 13 calcium or calcium and phospholipid binding proteins with high biological and structural homology. Annexin-1 (ANXA1) is the frst characterized member of the annexin family of proteins able to bind to cellular membranes in a calcium-dependent manner which promotes membrane fusion and is involved in endocytosis. Annexin A1 has anti-infammatory properties and inhibits phospholipase A2 activity. Annexin A1 can accumulate on internalized vesicles after EGF-stimulated endocytosis,suggesting that it may be required for a late stage in inward vesiculation.Phosphorylation by PKC, EGFR and Chak1 results in inhibition of annexin A1 function. Annexin A1 has also been identifed as one of the 'eat-me' signals on apoptotic cells to be recognised and ingested by phagocytes. Annexin A1, as an endogenous anti-infammatory mediator, has roles in many diverse cellular functions, such as membrane aggregation, infammation, phagocytosis,proliferation, apoptosis, and tumorigenesis and cancer development. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. AA Sequence: MAMVSEFLKQAWFIENEEQEYVQTVKSSKGGPGSAVSPYPTFNPSSDVAALHKAIMVKGVDEATIIDILTKRNNAQRQQIKAAYLQETGKPLDETLKKALTGHLEEVVLALLKTPAQFDADELRAAMKGLGTDEDTLIEILASRTNKEIRDINRVYREELKRDLAKDITSDTSGDFRNALLSLAKGDRSEDFGVNEDLADSDARALYEAGERRKGTDVNVFNTILTTRSYPQLRRVFQKYTKYSKHDMNKVLDLELKGDIEKCLTAIVKCATSKPAFFAEKLHQAMKGVGTRHKALIRIMVSRSEIDMNDIKAFYQKMYGISLCQAILDETKGDYEKILVALCGGN Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

Shipping Conditions:: Blue Ice