059513
Neurexin I
Size
£748.00
- SKU:
- 059513
- Purity:
- Purified by affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Chicken/Avian
- Immunogen:
- GST-tagged recombinant fragment corresponding to 51 amino acids from the C-terminal, cytoplasmic domain of human Neurexin-1. Species Sequence Homology: rat
- Format:
- Supplied as a liquid in 50mM HEPES, pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 20% glycerol.
- Extra Details:
- Neurexin-1 (UniProt: Q9CS84; also known as Neurexin I-alpha, Neurexin-1-alpha) is encoded by the Nrxn1 (also known as Kiaa0578) gene (Gene ID: 18189) in murine species. Neurexins are a large family of proteins that act as neuronal cell-surface receptors. Their function appears to be isoform-specific. They are heavily glycosylated and processed neuronal transmembrane cell surface proteins that are involved in cell recognition and adhesion. Neurexin-1 (the isoform) is a single-pass type I presynaptic membrane glycoprotein that is synthesized with a signal peptide that is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 31-1438), a transmembrane domain (aa 1439-2459), and a cytoplasmic domain (aa 1460-1514). It has a long N-terminus with six laminin G-like domains and play an important role in synaptic signal transmission. It is involved in the regulation of calcium channel activity and Ca2+-triggered neurotransmitter release at synapses and at neuromuscular junctions. It binds to neuroligins through an extracellular laminin neurexin sex-hormone-binding protein (LNS) domain. The neuroligin neurexin complex, a heterophilic adhesion system, promotes assembly and maturation of synapses through bidirectional signaling. In this complex, postsynaptic neuroligins interact trans-synaptically with presynaptic neurexins. This pan-neurexin antibody recognizes - and β-neurexins. Seven isoforms of Neurexin-1 have been described that are produced by alternate promoter usage and alternative splicing. Applications: Suitable for use in Western Blot, Immunofluorescence and Electron microscopy. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 detected Neurexin-1 in human brain tissue lysate. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice