Keratin 15 (KRT15, CK15, CK-15, Cytokeratin-15, K15, K1CO, Keratin, type I cytoskeletal 15, KRTB) (Preservative Free)

Size

£793.00

SKU:: 059511
Application:: IHC-Fr
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptide corresponding to 17 residues from the C-terminal of human cytokeratin 15. Cellular Localization: Cytoplasmic.
Clone:: LHK15
Format:: Supplied as a liquid in PBS. No preservative added.
Extra Details:: Keratin 15 antibody is a widely used reagent for exploring keratin 15, a type I intermediate filament protein expressed in epithelial cells. Keratin 15 is especially important as a marker of epithelial stem cell populations, most notably in the bulge region of the hair follicle. Because these cells contribute to tissue repair and regeneration, keratin 15 has become a focus in developmental biology, dermatology, and stem cell research. As part of the intermediate filament network, keratin 15 provides mechanical support that preserves cell shape and protects epithelial tissues under stress. Its filament assembly connects cells into a resilient structure that withstands stretching and external forces. Beyond structural roles, keratin 15 expression identifies regenerative populations that replenish skin and hair follicles following injury, making it an informative biomarker for regenerative capacity. 059511 has been extensively validated for its reliable detection of keratin 15 in epithelial stem cell niches. It is widely used to map bulge stem cells within the hair follicle and to examine their contribution to tissue maintenance. Its robust specificity ensures dependable results in both experimental and diagnostic settings, where identification of stem cell markers is critical. Keratin 15 expression has also been linked to oncology. In some cancers, its presence suggests that stem cell like populations may influence tumor initiation or progression. Research employing clone LHK15 has therefore been valuable not only in regenerative biology but also in cancer studies, where understanding stem cell markers provides insight into disease behavior and therapeutic strategies. By highlighting keratin 15 positive cells, researchers can better define the relationship between stemness and pathology. Applications: Suitable for use in Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry (FFPE): 0.1-0.2ug/ml for 30 min at RT. HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min. Optimal dilutions to be determined by the researcher. Positive Control: Human skin Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice