Green Fluorescent Protein (GFP)

Size

£639.00

SKU:: 059502
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Green Fluorescent Protein, expressed in E. coli. The epitope is dependent on amino acids 209-213 of eGFP, a region conserved in several GFP isoforms.
Clone:: 6H12
Format:: Supplied as a liquid in PBS, 5mM sodium azide, 50% glycerol.
Extra Details:: 059502 was made against a recombinant GFP construct originating from an Aequoria species which was engineered to improve spectral properties and prevent oligomerization (10). This form of GFP, referred to as AcGFP, is 94% identical to the eGFP developed by Tsien and coworkers and is the form of GFP inserted in the Clontech/Takara pAcGFP and related expression vectors. The antibody is strongly inhibited from binding to AcGFP by the peptide YLLPDNHYLSTQSALSKDNP and to a much lesser degree by the peptide SKDNPEKRDHMIYFGFVTAA. This suggests that the major determinant of antibody binding is the SKDNP and sequence N-terminal to this peptide. This region is amino acids 209-213 of GFP and corresponds to a turn between two β-strands of the β barrel GFP structure. This region is conserved in GFP variants and distinct from the homologous sequence seen in other fluorescent proteins. 059502 can be used to verify the expression, size and stability of both AcGFP and eGFP fusion proteins in western blotting experiments and to amplify GFP signals for ICC and IHC of transgenic animals. Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and Immunofluorescence. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:1000-1:5000 Immunofluorescence (IC): 1:1000-1:5000 Immunohistochemistry: 1:5000-1:10,000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice